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Immunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The mean serum Ig levels found for 6-month-old B6 mice were 0.22 mg/ml for IgM, 0.28 mg/ml for IgG1, 1.22 mg/ml for IgG2b, 0.18 mg/ml for IgG3, 0.075 mg/ml for IgA and about 0.7 mg/ml for IgG2a. This kit is for the quantitative measurement of mouse total IgG in mouse serum, plasma and supernatant from cell cultures.
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
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Standard curve
Example of mouse IgG (Total) standard curve in Assay Diluent #3.
Demonstration of the tested species specificity
Linearity
The concentrations of total IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse serum was 10^6 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3mg/mL in mouse serum.
#2 sample is undiluted samples are as follows: Mouse plasma was 10^6 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.2 mg/mL in mouse plasma.
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Protocol Diagram