Mouse Insulin ELISA Kit

insulin

Intended Uses: This INS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MouseINS. This ELISA kit for research use only, not for therapeutic or test applications! Principle of the Assay: INS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-INS antibody and an INS-HRP conjugate. The assay sample and buffer are incubated together with INS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INS concentration since INS from samples and INS-HRP conjugate compete for the anti-INS antibody binding site. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve.

Mouse

Quantitative Competitive

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

0.1 ng/mL

3630

P01308 [Other Products]