Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1)

Unconjugated,BSA Free

Protein A purified

Mouse

Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) is made to the N-Terminal decapeptide of Alpha smooth muscle isoform of actin and conjugated to KLH.

42 kDa.

1.0 mg/ml

0.02% Sodium Azide

Monoclonal

ACTA2

Flow (Intracellular) Flow Cytometry 0.5-1ug/million cellsImmunocytochemistry/ Immunofluorescence 0.5-1ug/mlImmunohistochemistry 1:10-1:500Immunohistochemistry-Frozen 1:600Immunohistochemistry-Paraffin 0.5-1.0ug/mlImmunoprecipitation 0.5-1ug/500ug protein lysateMultiplex Immunofluorescence 0.25ug/mLSimple Western 10 ug/mlWestern

Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Gp, Gt, Pm, Pm, Rb, ShSpecies Glossary

Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.