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Mouse Monoclonal MITF Antibody (MITF/915) - Azide and BSA Free
Mouse Monoclonal MITF Antibody (MITF/915) - Azide and BSA Free
品牌： Novus
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Protein A or G purified
反应种属：
Hu, Ca, Mu(-), Rt(-);Does not react with Mouse or Rat.
产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
宿主
Mouse
免疫原
Recombinant full-length human MITF protein (Uniprot: O75030)
简单描述
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45161).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
商品描述
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45161).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
同种型
IgG1 Kappa
克隆号
MITF/915
克隆类别
Monoclonal
纯度
Protein A or G purified
浓度
1 mg/ml
防腐剂
No Preservative
基因
MITF
应用
实验应用
IHC, ICC/IF
反应种属
Hu, Ca, Mu(-), Rt(-);Does not react with Mouse or Rat.
目标/特异性
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This monoclonal antibody recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
稀释度
Immunofluorescence 0.5 - 1.0 ug/mlImmunohistochemistry Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
背景
别名
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
背景
MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.
细胞定位
Nuclear
制备和贮存
保存方式
Store at -20 to -80C. Avoid freeze-thaw cycles.
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