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m/rLeptin Q-kit (1 Kit)
品牌： R&D Systems
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Product Summary
The Quantikine® Mouse/Rat Leptin Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse or rat Leptin in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse Leptin accurately. Results obtained for naturally occurring mouse or rat Leptin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse or rat Leptin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Tissue Homogenates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 152 | 421 | 1512 | 143 | 389 | 1469 |
| Standard Deviation | 5.4 | 8.58 | 41 | 11.1 | 30.5 | 87.2 |
| CV% | 3.6 | 2 | 2.7 | 7.8 | 7.8 | 5.9 |
Recovery
The recovery of mouse/rat Leptin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Media (n=4) | 109 | 98-117 |
| Mouse EDTA Plasma (n=4) | 96 | 89-110 |
| Mouse Heparin Plasma (n=4) | 90 | 80-105 |
| Mouse Serum (n=4) | 95 | 90-102 |
| Rat EDTA Plasma (n=4) | 106 | 99-113 |
| Rat Heparin Plasma (n=4) | 98 | 91-108 |
| Rat Serum (n=4) | 98 | 91-103 |
Linearity
To assess the linearity of the assay, four or more samples containing and/or spiked with high concentrations of mouse/rat Leptin in each matrix were diluted with calibrator diluent and assayed.
Scientific Data
Mouse/Rat Leptin Quantikine ELISA Kit Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Mouse Serum (10 uL), Rat Serum (25 uL), Mouse EDTA Plasma (10 uL), Rat EDTA Plasma (25 uL), Mouse Heparin Plasma (10 uL), Rat Heparin Plasma (25 uL)
Sensitivity
5.56 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma)
Specificity
Natural and recombinant mouse and rat Leptin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
背景
别名
FLJ94114,LEP,leptin (murine obesity homolog),leptin (obesity homolog, mouse),Leptin,OB,Obese protein,obese, mouse, homolog of,Obesity factor,OBOBS
背景
Background: Leptin/OB
Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.
Entrez Gene IDs:
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names:
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS
研究领域
制备和贮存
保存方式
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
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