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BD Pharmingen™ FITC Mouse Anti-Human CD20

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BD Pharmingen™ FITC Mouse Anti-Human CD20

品牌： BD Pharmingen

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参数

反应种属：

Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)

来源宿主：

Mouse C57BL/6 IgG2b, κ

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产品介绍

产品信息

耦联标记

FITC

抗原名称

CD20

宿主

Mouse C57BL/6 IgG2b, κ

免疫原

Human 6.16c1.3 B cell line

简单描述

The 2H7 monoclonal antibody specifically binds to CD20, encoded by the MS4A1 (Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37 kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation. This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes nor granulocytes, of baboon and both rhesus and cynomolgus macaque monkeys. The distribution on lymphocytes is similar to that seen with normal human donor lymphocytes, namely bright staining on B lymphocytes and weak reactivity on a small subset of CD3-positive T lymphocytes.

商品描述

2H7 The 2H7 monoclonal antibody specifically binds to CD20, encoded by the MS4A1 (Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37 kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation. This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes nor granulocytes, of baboon and both rhesus and cynomolgus macaque monkeys. The distribution on lymphocytes is similar to that seen with normal human donor lymphocytes, namely bright staining on B lymphocytes and weak reactivity on a small subset of CD3-positive T lymphocytes.

同种型

Mouse C57BL/6 IgG2b, κ

克隆号

克隆 2H7 (RUO)

产品详情

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:FITC

激发源:Blue 488 nm

最多激发源:494 nm

最多发射源:518 nm

应用

实验应用

Flow cytometry, Immunohistochemistry-frozen (Tested During Development)

推荐用量

20 µl

反应种属

Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)

目标/特异性

CD20

背景

别名

MS4A1; B1; Bp35; LEU-16; S7

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(3) 1. Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry. 1993; 14(2):193-204. (Biology). 2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. 3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

数据库链接

Entrez-Gene ID

931

参考图片

Flow cytometric analysis of CD20 expression on Rhesus macaque (Macaca mulatta) peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2b, κ Isotype Control (Cat. No. 556655; dashed line histogram) or FITC Mouse Anti-Human CD20 (Cat. No. 556632; solid line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.

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货号：

556632

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50Tst

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