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mTOR Antibody

1/5

mTOR Antibody

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

mTOR

来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2481 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50

分子量

289

内毒素水平

猪 , 马

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Pig,Horse,

目标/特异性

Specificity/Sensitivity

mTOR Antibody detects endogenous levels of total mTOR. It cross-reacts weakly with some other proteins based on Western analysis.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). 1.Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22. 2.Brown, E.J. et al. (1994) Nature 369, 756-8. 3.Sabatini, D.M. et al. (1994) Cell 78, 35-43. 4.Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. 5.Dennis, P.B. et al. (2001) Science 294, 1102-5. 6.Fang, Y. et al. (2001) Science 294, 1942-5. 7.Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. 8.Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23. 9.Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

2475

UniProt ID

P42345

参考图片

Simple Western™ analysis of lysates (1.0 mg/mL) from Hela cells using mTOR Antibody #2972. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.

Western blot analysis of extracts from control HEK293 cells (Lane 1) or HEK293 cells with a targeted mutation in the gene encoding mTOR (Lane 2) using mTOR Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The change in mTOR molecular weight in the mutated HEK293 cells confirms the specificity of the antibody for mTOR.

Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or mTOR siRNA (+). mTOR was detected using mTOR Antibody #2972, and eIF4B was detected using eIF4B Antibody #3592. The mTOR Antibody confirms silencing of mTOR expression, and the eIF4B Antibody is used to control for loading and siRNA specificity.

Western blot analysis of extracts from 293 cells (starved for 16 hours), untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) antibody #2971 (upper) or mTOR Antibody (lower).

Western blot analysis of extracts from various cell lines using mTOR Antibody.

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货号：

2972S

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100ul

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