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Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb

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Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

mTOR

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50
Immunofluorescence (Immunocytochemistry)	1:50 - 1:100

同种型

Rabbit IgG

分子量

289

内毒素水平

大鼠, 鸡 , 猪 , 马

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Rat,Chicken,Pig,Horse,

目标/特异性

Specificity/Sensitivity

Phospho-mTOR (Ser2448) (D9C2) XP Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). 1.Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22. 2.Brown, E.J. et al. (1994) Nature 369, 756-8. 3.Sabatini, D.M. et al. (1994) Cell 78, 35-43. 4.Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. 5.Dennis, P.B. et al. (2001) Science 294, 1102-5. 6.Fang, Y. et al. (2001) Science 294, 1942-5. 7.Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. 8.Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23. 9.Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

2475

UniProt ID

P42345

参考图片

Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (#9904, 10 nM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (upper) or mTOR (7C10) Rabbit mAb #2983.

Simple WesternTM analysis of lysates (0.1 mg/mL) from MCF-7 cells treated with insulin (100nM, 10 minutes) using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.

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货号：

5536T

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