Multiubiquitin Chain Monoclonal Antibody (Clone FK2)

Mouse

Crude preparation of polyubiquitinated-lysozyme

For immunochemical analysis of multiubiquitin chain

Ubiquitin is a small protein composed of 76 amino acids and having a molecular weight of 8.6 kD. It exists in all eukaryotic cells and is responsible for numerous functions in the cell by post-translational modifications of substrate proteins (ubiquitination). This process is important to numerous cellular processes including, but not limited to, apoptosis, cell cycle and division, DNA transcription, immune response, modulation of cell surface receptors, and viral infections. Clone FK2 is the most widely used ubiquitin antibody. This clone reacts with mono- and polyubiquitinated conjugates, but does not react with free ubiquitin.1,2

IgG1κ

FK2

ELISA, IHC, WB

(-) Free ubiquitin

≥ 3 years

1.Takada, K.,Nasu, H.,Hibi, N., et al. Serum concentrations of free ubiquitin and multiubiquitin chains. Clinical Chemistry 43(7), 1188-1195 (1997).
2.Fujimuro, M.,Sawada, H. and Yokosawa, H. Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins. FEBS Letters 349(2), 173-180 (1994).