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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu416 of human c-Myc.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (top, positive) versus wild-type 293T cells (bottom, negative) using Myc-Tag (E7F9B) Mouse mAb (green; mouse IgG1), RCAS1 (D2B6N) XP® Rabbit mAb #12290 (yellow; rabbit IgG), and Lamin A/C (4C11) Mouse mAb #4777 (red; mouse IgG2a). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing FOXO3-Myc using Myc-Tag (E7F9B) Mouse mAb as the primary antibody. Various anti-mouse isotype specific antibodies (upper) and Anti-mouse IgG, HRP-linked Antibody #7076 (lower) were used as secondary antibodies.
Western blot analysis of recombinant human SYN3-MYC/DYKDDDDK at various concentrations using, Myc-Tag (E7F9B) Mouse mAb.
Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (left, positive) versus wild-type 293T cells (right, negative) using Myc-Tag (E7F9B) Mouse mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).