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Myc-Tag (E7F9B) Mouse mAb
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Myc-Tag (E7F9B) Mouse mAb

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品牌: CST
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来源宿主:
Mouse
产品介绍
产品介绍
产品信息
荧光素标记
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu416 of human c-Myc.

宿主
Mouse
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunofluorescence (Immunocytochemistry)1:200
同种型
Mouse IgG1
应用
目标/特异性

Specificity/Sensitivity

Myc-Tag (E7F9B) Mouse mAb detects detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (E7F9B) Mouse mAb detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.The antibody may cross-react with c-myc protein.

Species Reactivity:

All Species Expected

敏感性
Transfected Only
背景
背景
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems (1). 1.Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

参考图片

Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (top, positive) versus wild-type 293T cells (bottom, negative) using Myc-Tag (E7F9B) Mouse mAb (green; mouse IgG1), RCAS1 (D2B6N) XP® Rabbit mAb #12290 (yellow; rabbit IgG), and Lamin A/C (4C11) Mouse mAb #4777 (red; mouse IgG2a). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing FOXO3-Myc using Myc-Tag (E7F9B) Mouse mAb as the primary antibody. Various anti-mouse isotype specific antibodies (upper) and Anti-mouse IgG, HRP-linked Antibody #7076 (lower) were used as secondary antibodies.

Western blot analysis of recombinant human SYN3-MYC/DYKDDDDK at various concentrations using, Myc-Tag (E7F9B) Mouse mAb.

Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (left, positive) versus wild-type 293T cells (right, negative) using Myc-Tag (E7F9B) Mouse mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

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货号:
92013T
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询价
20μl
100ul
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