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Myc-Tag (71D10) Rabbit mAb
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Myc-Tag (71D10) Rabbit mAb

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品牌: CST
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反应种属:
All
来源宿主:
Rabbit
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Myc-Tag
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Simple Western™1:10 - 1:50
Immunoprecipitation1:200
Immunofluorescence (Immunocytochemistry)1:200 - 1:400
Flow Cytometry (Fixed/Permeabilized)1:50 - 1:100
同种型
Rabbit IgG
应用
反应种属
All
目标/特异性

Specificity/Sensitivity

Myc-Tag (71D10) Rabbit mAb detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (71D10) Rabbit mAb detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.The antibody may cross-react with c-myc protein by Flow cytometry and immunofluorescence assays.

Species Reactivity:

All Species Expected

敏感性
Transfected Only
背景
背景
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1). 1.Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

参考图片

Flow cytometric analysis of 293T cells, mock-transfected (blue) or transfected with a construct expressing Myc-Tag (green), using Myc-Tag (71D10) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing Myc-Bcl-2 (+), using Bcl-2 Antibody #2872 (left) and Myc-Tag (71D10) Rabbit mAb (right).

Western blot analysis of recombinant human SYN3-MYC/DYKDDDDK at various concentrations using, Myc-Tag (71D10) Rabbit mAb.

Simple Western™ analysis of lysates (1.0 mg/mL) from 293T cells transfected with ATG13-MYC-DDK-ULK1 using Myc-Tag (71D10) Rabbit mAb #2278. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (left) versus wild-type 293 cells (right) using Myc-Tag (71D10) Rabbit mAb (green). Blue = DAPI #4083 (fluorescent DNA dye).

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货号:
2278S
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