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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Myc-Tag
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
宿主
Mouse
简单描述
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry in cells transfected with Myc-tagged protein.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
同种型
Mouse IgG2a kappa
克隆号
9B11
应用
反应种属
All
目标/特异性
Specificity/Sensitivity
Myc-Tag (9B11) Mouse mAb (PE Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (9B11) Mouse mAb (PE Conjugate) detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. The antibody may cross-react with c-myc protein.
Species Reactivity:
All Species Expected
敏感性
Transfected Only
背景
背景
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems.
1.Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
参考图片
Flow cytometric analysis of COS-7 cells, untreated (blue) or Myc transfected (green), using Myc-Tag (9B11) Mouse mAb (PE Conjugate).
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