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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #34664.
参考图片
Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or GFP-transfected (right), using GFP (D5.1) Rabbit mAb.
Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP (D5.1) Rabbit mAb.
Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells transfected with GFP using GFP (D5.1) Rabbit mAb #2956. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.