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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human nanog protein.
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652. The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Specificity/Sensitivity
Species Reactivity:
Human
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
CUT&Tag was performed with NCCIT cells and Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated), using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across chromosome 3 (upper), including SOX2 (lower), a known target gene of Nanog (see our ChIP-qPCR figure).
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677 or Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated), using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. Oct-4 and Nanog are known to associate with each other on chromatin. The figure shows binding of both Oct-4 and Nanog across POU5F1/Oct-4, a known target gene of both Oct-4 and Nanog (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product datasheet.
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and 10 µl of either Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677 or Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated), using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. Oct-4 and Nanog are known to associate with each other on chromatin. The figure shows binding of both Oct-4 and Nanog across chromosome 6 (upper), including POU5F1/Oct-4 (lower), a known target gene of both Oct-4 and Nanog (see additional figure containing ChIP-qPCR data).
Chromatin immunoprecipitations were performed with cross-linked chromatin from NTERA-2 cells and Nanog, Oct-4 and Sox2 antibodies, or Normal Rabbit IgG, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
CUT&RUN was performed with NCCIT cells and Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated), using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across Oct-4/POU5F1, a known target gene of Nanog (see additional figure containing CUT&RUN-qPCR data).
CUT&RUN was performed with NCCIT cells and Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated), using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 6 (upper), including Oct-4/POU5F1 (lower), a known target gene of Nanog (see additional figure containing CUT&RUN-qPCR data).
CUT&RUN was performed with NCCIT cells and either Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and human ITM2A promoter primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
CUT&Tag was performed with NCCIT cells and Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated), using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across SOX2, a known target gene of Nanog (see our ChIP-qPCR figure).