Napsin A Aspartic Peptidase (NAPSA) BioAssay™ ELISA Kit (Human)

Napsin A Aspartic Peptidase (NAPSA) BioAssay™ ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Napsin A Aspartic Peptidase (NAPSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Napsin A Aspartic Peptidase (NAPSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Napsin A Aspartic Peptidase (NAPSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Napsin A Aspartic Peptidase (NAPSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

46.875-3000pg/ml

Tests96 Colorimetric Serum, plasma and other biological fluids.

<18.22pg/ml

UN2796 PGII

38220000