BD Phosflow™ BV421 Mouse Anti-Human NF-κB p65 (pS529)

BV421

NF-κB p65 (pS529)

Mouse BALB/c IgG2b, κ

Phosphorylated Human NF-κB p65 Peptide

The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of the human NF- κ B p65 subunit. Nuclear factor κ B (NF- κ B) is a ubiquitously expressed transcription factor that regulates the expression of many other genes. It is crucial for cellular responses to a variety of stimuli including stress and microbial pathogens that lead to immunity, inflammation, proliferation, differentiation, survival, apoptosis, and tumorigenesis. The most studied NF- κ B complex consists of the p50 (also known as NF- κ B1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor I κ B and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I κ B. This complex prevents nuclear translocation and activity of NF- κ B. In response to stimuli such as cytokines, LPS, DNA damage, and microbial infections, I κ B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I κ B/NF- κ B complex, allowing the free heterodimeric NF- κ B to translocate to the nucleus. Furthermore, optimal activation of NF- κ B requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF- κ B dimers bind to the κ B sites within promoters and enhancers and function as transcriptional regulators. The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

K10-895.12.50 The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of the human NF- κ B p65 subunit. Nuclear factor κ B (NF- κ B) is a ubiquitously expressed transcription factor that regulates the expression of many other genes. It is crucial for cellular responses to a variety of stimuli including stress and microbial pathogens that lead to immunity, inflammation, proliferation, differentiation, survival, apoptosis, and tumorigenesis. The most studied NF- κ B complex consists of the p50 (also known as NF- κ B1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor I κ B and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I κ B. This complex prevents nuclear translocation and activity of NF- κ B. In response to stimuli such as cytokines, LPS, DNA damage, and microbial infections, I κ B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I κ B/NF- κ B complex, allowing the free heterodimeric NF- κ B to translocate to the nucleus. Furthermore, optimal activation of NF- κ B requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF- κ B dimers bind to the κ B sites within promoters and enhancers and function as transcriptional regulators. The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Mouse BALB/c IgG2b, κ

克隆 K10-895.12.50 (RUO)

Intracellular staining (flow cytometry) (Routinely Tested)

5 µl

Human (QC Testing)

NF-κB p65 (pS529)

RELA; REL-A; NFKBp65; NFKB3; Transcription factor p65; TF65

Phosphate

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

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