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p75NTR (D4B3) XP ® Rabbit mAb

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p75NTR (D4B3) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

p75NTR

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg198 of human p75NTR protein. This antibody is predicted to bind the extracellular amino-terminal region of p75NTR protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunofluorescence (Frozen)	1:1600 - 1:3200
Immunofluorescence (Immunocytochemistry)	1:1600 - 1:3200
Flow Cytometry (Live)	1:200 - 1:800

同种型

Rabbit IgG

分子量

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human,Mouse,Rat,

目标/特异性

Specificity/Sensitivity

p75NTR (D4B3) XP Rabbit mAb recognizes endogenous levels of total p75NTR protein. Nonspecific cytoplasmic staining is observed in fixed frozen mouse spleen and colon by immunofluorescence.

Species Reactivity:

Human, Mouse, Rat

敏感性

Endogenous

背景

The p75 neurotrophin receptor (p75NTR), a member of the TNF receptor superfamily, is distinguished by multiple cysteine-rich ligand-binding domains, a single transmembrane sequence, and a noncatalytic cytoplasmic domain (1). p75NTR displays paradoxical functions when acting alone or with other receptor proteins. Working in concert with Trk receptors, p75NTR recognizes neurotrophins and transmits trophic signals into the cell. Both p75NTR and TrkA are required to activate PI3K-Akt signaling, while TrkA can individually activate the MAP kinase pathway. In contrast, p75NTR, possibly through JNK, ensures appropriate apoptosis of injured neurons and improperly targeted neonatal neurons (2).The p75NTR protein undergoes sequential cleavage similar to APP and Notch. First, α-secretase removes the p75NTR ectodomain, eliminating ligand-mediated signaling. At this point, the membrane-tethered cleavage product can still fine-tune Trk-mediated trophic actions. γ-secretase cleaves within the transmembrane domain to liberate the cytoplasmic tail from its membrane anchor and allow the p75NTR intracellular domain to translocate to the nucleus (3,4). 1.Chao, M.V. (2003) Nat. Rev. Neurosci. 4, 299-309. 2.Nykjaer, A. et al. (2005) Curr. Opin. Neurobiol. 15, 49-57. 3.Kanning, K.C. et al. (2003) J. Neurosci. 23, 5425-5436. 4.Jung, K.M. et al. (2003) J. Biol. Chem. 278, 42161-42169.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #46333.

数据库链接

Entrez-Gene ID

4804

UniProt ID

P08138

参考图片

Flow cytometric analysis of live DLD-1 cells (blue, negative) and SK-N-MC cells (green, positive) using p75NTR (D4B3) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from PC-3 cells, untreated (-) or ibuprofen-treated (2 mM, 24 hr) (+), and SW480 cells (-) using p75NTR (D4B3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Immunoprecipitation of p75NTR from SW-480 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is p75NTR (D4B3) XP® Rabbit mAb. Western blot was performed using p75NTR (D4B3) XP® Rabbit mAb.

Confocal immunofluorescent analysis of fixed frozen mouse optic tract labeled with p75NTR (D4B3) XP® Rabbit mAb (green) and DAPI #8961 (blue).

Confocal immunofluorescent analysis of fixed frozen developing mouse E12 spinal cord and somites labeled with p75NTR (D4B3) XP® Rabbit mAb (left, green), DyLight™ 554 Phalloidin #13054 (right, red), and DAPI #8961 (right, blue).

Confocal immunofluorescent analysis of SK-N-MC cells using p75NTR (D4B3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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货号：

8238T

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