




Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NONO protein.


Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunofluorescence (Frozen) | 1:100 - 1:400 |
Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |





Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey




Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


参考图片
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Actinomycin D #15021 (5 µg/mL, 2 hr; right), using NONO (E8Q3E) Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).
Western blot analysis of extracts from 293T, transfected with control siRNA (-) or NONO siRNA (+), using NONO (E8Q3E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using NONO (E8Q3E) Rabbit mAb.
Immunoprecipitation of NONO protein from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NONO (E8Q3E) Rabbit mAb. Western blot analysis was performed using NONO (E8Q3E) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Confocal immunofluorescent analysis of formaldehyde fixed frozen mouse testis labeled with NONO (E8Q3E) Rabbit mAb (green) and DAPI #4038 (blue).
Confocal immunofluorescent analysis of HCT 116 cells, transfected with control siRNA (left) or NONO siRNA (right), using NONO (E8Q3E) Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).