NONO (E8Q3E) Rabbit mAb

Non-POU domain-containing octamer-binding protein (NONO), also referred to as p54nrb, is a DNA and RNA-binding protein that is involved in several nuclear processes. NONO binds to the conventional octamer duplex of double-stranded DNA, and can additionally bind to single-stranded DNA and RNA at a location independent of the duplex site (1). NONO belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with paraspeckle protein 1 (PSPC1) and splicing factor, proline- and glutamine-rich (PSF/SFPQ) (2). NONO, PSPC1, and PSF/SFPQ associate together along with long nonprotein-coding RNA NEAT1 to form ribonucleic protein bodies referred to as paraspeckles, which play a role in gene expression and regulation (2). NONO heterodimerizes with PSF/SFPQ to process pri-miRNA prior to presentation to the Drosha complex (3). The NONO/PSF heterodimer can also stabilize paired DNA ends and promote DNA double-strand break repair by canonical non-homologous end rejoining (4). NONO also forms a complex with PSF/SFPQ and DExH-box helicase 9 (DHX9) that produces a long isoform of BIN1, promoting carcinogenesis in hepatocellular carcinoma (5). In breast cancer, NONO regulates pre-mRNA splicing and binding to mRNAs of cell proliferation-related genes, regardless of hormone sensitivity (6). NONO has also been hypothesized to play a role in heart disease, and a recent study in mice showed that NONO deficiency led to heart defects by fibroblast hyperproliferation (7). 1.Yang, Y.S. et al. (1993) Mol Cell Biol 13, 5593-603. 2.Bond, C.S. and Fox, A.H. (2009) J Cell Biol 186, 637-44. 3.Jiang, L. et al. (2017) Nat Struct Mol Biol 24, 816-24. 4.Jaafar, L. et al. (2017) Nucleic Acids Res 45, 1848-59. 5.Hu, Z. et al. (2019) Hepatology, doi: 10.1002/hep.31062. 6.Iino, K. et al. (2020) Cancer Sci 111, 148-59. 7.Xu, X. et al. (2019) J Mol Cell Cardiol 137, 46-58.