老带新
NONO (E8Q3E) Rabbit mAb
1/6

NONO (E8Q3E) Rabbit mAb

分享
品牌: CST
pdf 下载产品说明书
pdf 下载COA
pdf 下载SDS
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
分子量:
60
反应种属:
Human,Mouse,Rat,Monkey,
展开
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
NONO
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NONO protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunoprecipitation1:50
Immunofluorescence (Frozen)1:100 - 1:400
Immunofluorescence (Immunocytochemistry)1:100 - 1:400
同种型
Rabbit IgG
分子量
60
研究领域
RNA 调节与翻译调控
应用
反应种属
Human,Mouse,Rat,Monkey,
目标/特异性

Specificity/Sensitivity

NONO (E8Q3E) Rabbit mAb recognizes endogenous levels of total NONO protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性
Endogenous
背景
背景
Non-POU domain-containing octamer-binding protein (NONO), also referred to as p54nrb, is a DNA and RNA-binding protein that is involved in several nuclear processes. NONO binds to the conventional octamer duplex of double-stranded DNA, and can additionally bind to single-stranded DNA and RNA at a location independent of the duplex site (1). NONO belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with paraspeckle protein 1 (PSPC1) and splicing factor, proline- and glutamine-rich (PSF/SFPQ) (2). NONO, PSPC1, and PSF/SFPQ associate together along with long nonprotein-coding RNA NEAT1 to form ribonucleic protein bodies referred to as paraspeckles, which play a role in gene expression and regulation (2). NONO heterodimerizes with PSF/SFPQ to process pri-miRNA prior to presentation to the Drosha complex (3). The NONO/PSF heterodimer can also stabilize paired DNA ends and promote DNA double-strand break repair by canonical non-homologous end rejoining (4). NONO also forms a complex with PSF/SFPQ and DExH-box helicase 9 (DHX9) that produces a long isoform of BIN1, promoting carcinogenesis in hepatocellular carcinoma (5). In breast cancer, NONO regulates pre-mRNA splicing and binding to mRNAs of cell proliferation-related genes, regardless of hormone sensitivity (6). NONO has also been hypothesized to play a role in heart disease, and a recent study in mice showed that NONO deficiency led to heart defects by fibroblast hyperproliferation (7). 1.Yang, Y.S. et al. (1993) Mol Cell Biol 13, 5593-603. 2.Bond, C.S. and Fox, A.H. (2009) J Cell Biol 186, 637-44. 3.Jiang, L. et al. (2017) Nat Struct Mol Biol 24, 816-24. 4.Jaafar, L. et al. (2017) Nucleic Acids Res 45, 1848-59. 5.Hu, Z. et al. (2019) Hepatology, doi: 10.1002/hep.31062. 6.Iino, K. et al. (2020) Cancer Sci 111, 148-59. 7.Xu, X. et al. (2019) J Mol Cell Cardiol 137, 46-58.
研究领域
RNA 调节与翻译调控
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接
Entrez-Gene ID
4841
UniProt ID
Q15233

参考图片

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Actinomycin D #15021 (5 µg/mL, 2 hr; right), using NONO (E8Q3E) Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).

Western blot analysis of extracts from 293T, transfected with control siRNA (-) or NONO siRNA (+), using NONO (E8Q3E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from various cell lines using NONO (E8Q3E) Rabbit mAb.

Immunoprecipitation of NONO protein from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NONO (E8Q3E) Rabbit mAb. Western blot analysis was performed using NONO (E8Q3E) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

Confocal immunofluorescent analysis of formaldehyde fixed frozen mouse testis labeled with NONO (E8Q3E) Rabbit mAb (green) and DAPI #4038 (blue).

Confocal immunofluorescent analysis of HCT 116 cells, transfected with control siRNA (left) or NONO siRNA (right), using NONO (E8Q3E) Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).

声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
货号:
10162S
一键复制
询价
供应商现货
20μl
100ul
选择数量
当前规格1件起购 
配送至
预计5-6个工作日送达,快递: 免运费,若需干冰额外收费