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The purified antibody is not directed against any known antigen. It was isolated from pooled mouse sera.
Normal Mouse IgG is an unconjugated mouse polyclonal antibody that is routinely used as a non-specific IgG control and works optimally with the Cell Signaling Technology® CUT&Tag protocol.
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652. The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody. This product is stable for 24 months when stored at –20°C.
参考图片
CUT&Tag was performed with HCT 116 cells and Rpb1 CTD (4H8) Mouse mAb #2629 or Normal Mouse IgG, using CUT&Tag Assay Kit #77552. DNA libraries were prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across chromosome 12 (upper), including GAPDH (lower), a known target gene of Rpb1.
CUT&RUN was performed with HeLa cells and Rpb1 CTD (4H8) Mouse mAb #2629 or Normal Mouse IgG, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across GAPDH, a known target gene of Rpb1 (see additional figure containing CUT&RUN-qPCR data).
CUT&RUN was performed with HeLa cells and Rpb1 CTD (4H8) Mouse mAb #2629 or Normal Mouse IgG, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 12 (upper), including GAPDH (lower), a known target gene of Rpb1 (see additional figure containing CUT&RUN-qPCR data).
CUT&RUN was performed with HeLa cells and Rpb1 CTD (4H8) Mouse mAb #2629 or Normal Mouse IgG, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RPL30 Exon 3 Primers #7014, human GAPDH exon 1 primers, and SimpleChIP® Human MyoD1 Exon 1 Primers #4490. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
CUT&Tag was performed with HCT 116 cells and Rpb1 CTD (4H8) Mouse mAb #2629 or Normal Mouse IgG, using CUT&Tag Assay Kit #77552. DNA libraries were prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across GAPDH, a known target gene of Rpb1.