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产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
抗原名称
Notch1
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2498 of human Notch1.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |
同种型
Rabbit IgG
分子量
120, 300
研究领域
癌症,发育生物学与干细胞研究,神经科学
应用
反应种属
Human,Mouse,Rat,
目标/特异性
Specificity/Sensitivity
Notch1 (D6F11) XP Rabbit mAb detects intracellular epitopes between 2450 and 2550 amino acids of human Notch1. It recognizes both the full-length (~300 KDa) and the NTM region (~120 KDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). The antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases.
Species Reactivity:
Human, Mouse, Rat
敏感性
Endogenous
背景
背景
Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4).
1.Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2.Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3.Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4.Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
研究领域
癌症,发育生物学与干细胞研究,神经科学
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
4851
UniProt ID
P46531
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