PathScan ® Cleaved Notch1 (Val1744) Sandwich ELISA Kit

1/2

PathScan ® Cleaved Notch1 (Val1744) Sandwich ELISA Kit

品牌： CST

下载产品说明书

下载COA

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

实验应用：

产品介绍

产品信息

产品详情

Developmental Biology

简单描述

PathScan® Cleaved Notch1 (Val1744) Sandwich ELISA Kit from Cell Signaling Technology is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Notch1 when cleaved at Val1744. A Notch1 rat antibody has been coated onto the microwells. After incubation with cell lysates, Notch1 protein is captured by the coated antibody. Following extensive washing, a cleaved Notch1 (Val1744) Rabbit detection antibody is added to detect the captured Notch1 protein. Anti-Rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of cleaved Notch1 (Val1744).

*Antibodies in this kit are custom formulations specific to kit.

组合货号

7194CA&9803S

研究领域

发育生物学与干细胞研究,神经科学,

应用

实验应用

ELISA

反应种属

Human

目标/特异性

Specificity/Sensitivity

PathScan Cleaved Notch1 (Val1744) Sandwich ELISA Kit #7194 detects endogenous levels of Notch1 protein when cleaved at Val1744 as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

背景

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4). 1.Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6. 2.Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6. 3.Schroeter, E.H. et al. (1998) Nature 393, 382-6. 4.Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.

研究领域

发育生物学与干细胞研究,神经科学,

翻译后修饰

cleaved

数据库链接

Entrez-Gene ID

4851

UniProt ID

P46531

参考图片

Figure 2. The relationship between the protein concentration of lysates from Jurkat cells, untreated or treated with γ secretase inhibitor, and the absorbance at 450 nm using the PathScan® Cleaved Notch1 (Val1744) Sandwich ELISA Kit #7194 is shown.

Figure 1. Jurkat cells express cleaved Notch1 (Val1744) as detected by the PathScan® Cleaved Notch1 (Val1744) Sandwich ELISA Kit #7194. Treatment with γ secretase inhibitor inhibits the cleavage of Notch1. Jurkat cells were treated with 25 μM γ secretase inhibitor for 96 hours. The absorbance readings at 450 nm are shown on the top, while the corresponding western blots using Notch1 (D1E11) XP® Rabbit mAb #3608 (left panel) or Cleaved Notch1 (Val1744) Antibody #2421 (right panel) are shown on the bottom.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

7194C

一键复制

询价

1Kit

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价