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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2378 of human Notch2 protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:250 - 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:3200 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #17635.
参考图片
Flow cytometric analysis of fixed and permeabilized SW620 cells (red, low expression) and 293 cells (blue, positive) cells using Notch2 (D76A6) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of extracts from 293 and SK-MEL-5 cells using Notch2 (D76A6) XP® Rabbit mAb. The full-length (FL) Notch2 protein and the transmembrane/intracellular region (NTM) are indicated.
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using Notch2 (D76A6) XP® Rabbit mAb performed on the Leica BOND RX.
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using Notch2 (D76A6) XP® Rabbit mAb performed on the Leica BOND RX.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using Notch2 (D76A6) XP® Rabbit mAb performed on the Leica BOND RX.
Immunohistochemical analysis of paraffin-embedded normal human placenta using Notch2 (D76A6) XP® Rabbit mAb performed on the Leica BOND RX.
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Notch2 (D76A6) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Notch2 (D76A6) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Notch2 (D76A6) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Notch2 (D76A6) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human placenta using Notch2 (D76A6) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human testis using Notch2 (D76A6) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma (left) or endometrioid adenocarcinoma (right) using Notch2 (D76A6) XP® Rabbit mAb (top) or Notch2 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human Notch2. Both epitopes are contained within the intracellular domain and the nuclear staining represents active Notch2. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using Notch2 (D76A6) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded 293T cell pellet (left, positive) or IMR-32 cell pellet (right, negative) using Notch2 (D76A6) XP® Rabbit mAb.
Confocal immunofluorescent analysis of SNB19 cells using Notch2 (D76A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).