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NADH Dehydrogenase, Quinone 1 (NQO1) BioAssay™ ELISA Kit (Rat)
NADH Dehydrogenase, Quinone 1 (NQO1) BioAssay™ ELISA Kit (Rat)
品牌： Usbiological
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咨询实验应用：
Sandwich ELISA
产品介绍
产品介绍
产品信息
简单描述
NADH Dehydrogenase, Quinone 1 (NQO1) BioAssay™ ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase, Quinone 1 (NQO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase, Quinone 1 (NQO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase, Quinone 1 (NQO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of NADH Dehydrogenase, Quinone 1 (NQO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品类型
Sandwich ELISA
应用
实验应用
Sandwich ELISA
检测范围
0.313-20ng/ml
检测类型
Colorimetric
样本类型以及上样量
Tissue homogenates, cell lysates and other biological fluids.
敏感性
<0.126ng/ml
制备和贮存
保存方式
Shipping Temp:Blue Ice
Storage Temp:4°C/-20°C
Storage and StabilityDesiccation recommended. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt. Store all other reagents at 4°C. Stable for 6 months after receipt of order.
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