NUCLEOLAR-ID® Green detection kit

Protect from light. Avoid freeze/thaw cycles.

The NUCLEOLAR-ID® Green detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Compatible with common live-cell nuclear counterstains (Hoechst 33342, Draq5™, Vybrant® DyeCycle™ Ruby, NUCLEAR-ID® Red dyes) High resistance to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods Validated for utility in live cell imaging applications, demonstrating appropriate response to treatment with well-characterized organelle-perturbation agents Stringently manufactured, to control and eliminate non-specific assay artifacts Enzo  Life Sciences’ NUCLEOLAR-ID® Green Detection Kit contains a proprietary dye suitable for live-cell staining of nucleoli. The dye allows examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for visualizing nucleoli in living cells. The dye in the kit is resistant to photobleaching, facilitating its use in imaging applications. This nucleolar dye may be used in conjunction with common live-cell nuclear counterstains such as Hoechst 33342, Draq5™, Vybrant® DyeCycle™ Ruby and Enzo’s NUCLEAR-ID® Red dye. A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition of transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.

Fluorescence microscopy, Fluorescent detection;Specifically designed for visualizing microscopically nucleoli in living cells.

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or  -80°C for longer term storage.