Numb (C29G11) Rabbit mAb

Numb contains an amino-terminal phosphotyrosine binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G protein-coupled receptor (GPCR) and protein kinase C (PKC) signaling (9). 1.Berdnik, D. et al. (2002) Dev. Cell 3, 221-231. 2.Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352. 3.Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104. 4.Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476. 5.Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155. 6.McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203. 7.Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145. 8.Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948. 9.Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.