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p16 INK4A (E5F3Y) Rabbit mAb

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p16 INK4A (E5F3Y) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of mouse p16 INK4A protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

同种型

Rabbit IgG

分子量

应用

目标/特异性

Specificity/Sensitivity

p16 INK4A (E5F3Y) Rabbit mAb recognizes endogenous levels of total p16 INK4A protein.

Species Reactivity:

Mouse

敏感性

Endogenous

背景

Members of the INK4 family of cyclin-dependent kinase inhibitors include p16 INK4A, p15 INK4B, p18 INK4C, and p19 INK4D. The INK4 family members inhibit cyclin-dependent kinases 4 and 6 (CDK4 and CDK6), causing cell cycle arrest in G1 phase. The INK4A-ARF-INK4B locus on chromosome 9p21, frequently lost in human cancer, encodes the INK4 family members p16 INK5A and p15 INK4B, as well as the unrelated protein, ARF (1).p16 INK4A expression, typically repressed in the absence of stress, is thought to drive cells into senescence, and p16 INK4A expression is a commonly used marker of senescent cells (2,3). p16 INK4A protein expression is often altered in human cancer, and high expression is currently used as a predictive biomarker in cervical cancer (3-5). 1.Kim, W.Y. and Sharpless, N.E. (2006) Cell 127, 265-75. 2.LaPak, K.M. and Burd, C.E. (2014) Mol Cancer Res 12, 167-83. 3.Romagosa, C. et al. (2011) Oncogene 30, 2087-97. 4.Ishikawa, M. et al. (2006) Int J Gynecol Cancer 16, 347-53. 5.Queiroz, C. et al. (2006) Pathol Res Pract 202, 77-83.

研究领域

癌症,细胞生物学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

12578

UniProt ID

P51480

参考图片

Immunoprecipitation of p16 INK4A protein from mouse A20 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is p16 INK4A (E5F3Y) Rabbit mAb. Western blot analysis was performed using p16 INK4A (E5F3Y) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing full-length mouse p16 INK4A protein (mINK4A-Myc/DDK; +) or full-length mouse p15 INK4B protein (mINK4B-Myc/DDK; +), using p16 INK4A (E5F3Y) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Western blot analysis of extracts from MEF cells, untreated (-) or treated with Doxorubicin #5927 (250 nM, 24 hours followed by 7 days without doxorubicin; +), using p16 INK4A (E5F3Y) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). p16 INK4A protein expression is induced by doxorubicin as expected.

Western blot analysis of extracts from various mouse cell lines using p16 INK4A (E5F3Y) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of p16 INK4A protein in NIH/3T3 cells is consistent with the predicted expression pattern.

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货号：

29271T

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询价

20μl

100ul

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