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BD Phosflow™ PE Mouse Anti-p38 MAPK (pT180/pY182)
品牌： BD Pharmingen
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咨询反应种属：
Human (QC Testing), Mouse,Rat (Tested in Development)
来源宿主：
Mouse IgG1, κ
产品介绍
产品使用步骤推荐 产品介绍
产品信息
荧光素标记
抗原名称
p38 MAPK (pT180/pY182)
宿主
Mouse IgG1, κ
免疫原
Phosphorylated Human p38 MAPK (pT180/pY182) Peptide
简单描述
Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the
M
itogen
A
ctivated
P
rotein
K
inases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAPKs include p38α (MAPK14), β (MAPK11), γ (MAPK12), and δ (MAPK13). These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the
M
AP
K
inase
K
inases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in multiple signal transduction pathways.
The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38α, β, γ, and δ.
商品描述
36/p38 (pT180/pY182)
Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the
M
itogen
A
ctivated
P
rotein
K
inases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAPKs include p38α (MAPK14), β (MAPK11), γ (MAPK12), and δ (MAPK13). These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the
M
AP
K
inase
K
inases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in multiple signal transduction pathways.
The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38α, β, γ, and δ.
同种型
Mouse IgG1, κ
克隆号
克隆 36/p38 (pT180/pY182) (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Mouse,Rat (Tested in Development)
目标/特异性
p38 MAPK (pT180/pY182)
背景
别名
MAPK14; p38 MAP kinase; p38 MAPK; RK; CSBP2
翻译后修饰
Phosphate
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Brunet A, Pouyssegur J. Identification of MAP kinase domains by redirecting stress signals into growth factor responses. Science. 1996; 272(5268):1652-1655. (Biology).
2. Han J, Lee JD, Bibbs L, Ulevitch RJ. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science. 1994; 265(5173):808-811. (Biology).
3. Winston BW, Chan ED, Johnson GL, Riches DW. Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages. J Immunol. 1997; 159(9):4491-4497. (Biology).
参考图片
Flow cytometric analysis of p38 MAPK (pT180/pY182) expression on human peripheral blood leukocytes. Treated human control cells (solid line histogram) and untreated human control cells (dashed line histogram) from the T Cell Kit Lyophilized Cells (Cat. No. 560760) were stained with PE Mouse Anti-p38 MAPK (pT180/pY182) (Cat. No. 612565). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable leukocytes.
Flow cytometric analysis of p38 MAPK (pT180/pY182) expression on human peripheral blood leukocytes. Treated human control cells (solid line histogram) and untreated human control cells (dashed line histogram) from the T Cell Kit Lyophilized Cells (Cat. No. 560760) were stained with PE Mouse Anti-p38 MAPK (pT180/pY182) (Cat. No. 612565). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable leukocytes.
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