p70 S6 Kinase, phosphorylated (Thr421/Ser424) (p70 S6K)

Affinity Purified Purified by Protein A affinity chromatography.

protein kinase, cAMP-dependent, catalytic, beta

Synthetic phosphopeptide corresponding to residues around Thr421/Ser424 of human p70 S6 kinase (KLH coupled).

p70 S6 kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal (1). Both isoforms lie on a mitogen-activated signaling pathway downstream of phosphoinositide 3 kinase (PI3K) and the target of rapamycin FRAP/mTOR. Activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, closely correlates with p70 kinase activity in vivo (2). Prior phosphorylation of Thr389 is required for phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (3,4). Phosphorylation is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI3 kinase inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,5,6). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2).

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Polyclonal

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

Human, Mouse, Rat

Anti -p70 S6 Kinase, phosphorylated (Thr421/Ser424) (p70 S6K)

100038053