PARP (46D11) Rabbit mAb (Sepharose ® Bead Conjugate)

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). 1.Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. 2.Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. 3.Cohen, G.M. (1997) Biochem. J. 326, 1-16. 4.Nicholson, D. W. et al. (1995) Nature 376, 37-43. 5.Tewari, M. et al. (1995) Cell 81, 801-809. 6.Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.