全部商品分类
1/4
用小程序，查商品更便捷
产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
抗原名称
PARP
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly623 of human PARP-1.
宿主
Rabbit
商品描述
Product Usage Information
For more information about the RBP-eCLIP service please visit Eclipsebio.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:200 |
| eCLIP | 1:200 |
同种型
Rabbit
分子量
116, 89
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
目标/特异性
Specificity/Sensitivity
PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP-1 and the large fragment (89 kDa) produced by caspase cleavage at Asp214. This antibody does not cross-react with PARP-2 and PARP-3.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
1.Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
2.Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
3.Cohen, G.M. (1997) Biochem. J. 326, 1-16.
4.Nicholson, D. W. et al. (1995) Nature 376, 37-43.
5.Tewari, M. et al. (1995) Cell 81, 801-809.
6.Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #17245.
数据库链接
Entrez-Gene ID
142
UniProt ID
P09874
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。