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PARP (46D11) Rabbit mAb

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PARP (46D11) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

PARP

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly623 of human PARP-1.

宿主

Rabbit

商品描述

Product Usage Information

For more information about the RBP-eCLIP service please visit Eclipsebio.

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:200
eCLIP	1:200

同种型

Rabbit

分子量

116, 89

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP-1 and the large fragment (89 kDa) produced by caspase cleavage at Asp214. This antibody does not cross-react with PARP-2 and PARP-3.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). 1.Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. 2.Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. 3.Cohen, G.M. (1997) Biochem. J. 326, 1-16. 4.Nicholson, D. W. et al. (1995) Nature 376, 37-43. 5.Tewari, M. et al. (1995) Cell 81, 801-809. 6.Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #17245.

数据库链接

Entrez-Gene ID

142

UniProt ID

P09874

参考图片

Enhanced cross-linking and immunoprecipitation (eCLIP) was performed with RNA from K-562 cells and PARP (46D11) Rabbit mAb using a protocol based on the RBP-eCLIP method from Eclipsebio. The figure shows binding across the GET4 transcript. Data is kindly provided by the laboratory of Dr. Gene Yeo and used with permission.

Western blot analysis of extracts from control HEK293 cells (lane 1) or PARP knockout HEK293 cells (lane 2) using PARP (46D11) Rabbit mAb #9532 (upper) or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the PARP knockout HEK293 cells confirms the specificity of the antibody for PARP.

Western blot analysis of extracts from THP-1 cells, untreated or treated with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb.

Simple Western™ analysis of lysates (1 mg/mL) from Jurkat cells treated with Etoposide (25 uM, 5 hours) using PARP (46D11) Rabbit mAb #9532. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

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9532T

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