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产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
抗原名称
PARP
来源纯化
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the caspase cleavage site in PARP. Antibodies are purified by protein A and peptide affinity chromatography.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
分子量
89, 116
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
目标/特异性
Specificity/Sensitivity
PARP Antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage. The antibody does not cross-react with related proteins or other PARP isoforms.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
1.Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
2.Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
3.Cohen, G.M. (1997) Biochem. J. 326, 1-16.
4.Nicholson, D. W. et al. (1995) Nature 376, 37-43.
5.Tewari, M. et al. (1995) Cell 81, 801-809.
6.Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
142
UniProt ID
P09874
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