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PARP Antibody

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PARP Antibody

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

PARP

来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the caspase cleavage site in PARP. Antibodies are purified by protein A and peptide affinity chromatography.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50

分子量

89, 116

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

PARP Antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage. The antibody does not cross-react with related proteins or other PARP isoforms.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). 1.Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. 2.Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. 3.Cohen, G.M. (1997) Biochem. J. 326, 1-16. 4.Nicholson, D. W. et al. (1995) Nature 376, 37-43. 5.Tewari, M. et al. (1995) Cell 81, 801-809. 6.Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

142

UniProt ID

P09874

参考图片

Simple Western™ analysis of lysates (1 mg/mL) from serum-starved HeLa cells treated with Staurosporine (1 uM, 3 hours) using PARP Antibody #9542. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Western blot analysis of extracts from NIH/3T3 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells, untreated or etoposide-treated (25 µM), using PARP Antibody.

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货号：

9542T

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