CST_PathScan ® Phospho-DDR1 (panTyr) Sandwich ELISA Kit_优宁维(univ)在线
PathScan ® Phospho-DDR1 (panTyr) Sandwich ELISA Kit
货号: 7863C 品牌: CST 反应种属:
Human
Human
应用:
ELISA
ELISA
规格: 1Kit
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价格: ¥6930.00

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产品信息
目标/特异性
PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit detects endogenous levels of tyrosine-phosphorylated DDR1 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
商品描述
The PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated DDR1 protein. A DDR1 rabbit antibody has been coated on the microwells. After incubation with cell lysates, DDR1 protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a phospho-tyrosine mouse mAb is added to detect captured tyrosine-phosphorylated DDR1 protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of DDR1 protein phosphorylated on tyrosine residues.
应用
实验应用
ELISA
反应种属
Human
背景
背景
盘状结构域受体 (DDR) 是一种受体酪氨酸激酶,它的胞外结构域中有 Discoindin 同源重复序列,并且它可通过与胞外基质胶原的结合被激活。到目前为止,已经确定了两种哺乳动物的 DDR:DDR1 和 DDR2 (1)。它们广泛表达于人体组织中,并有可能会对平滑肌细胞介导的胶原重塑产生作用 (2)。研究表明,在人体疾病中,DDR 的异常表达和信号转导与基质降解和重塑增强有关,如心血管疾病、肝纤维化和肿瘤侵袭 (1)。 Vogel, W. (1999) FASEB J 13 Suppl, S77-82. Ferri, N. et al. (2004) Am J Pathol 164, 1575-85.

参考图片

Figure 2: The relationship between protein concentration of phospho or nonphospho lysates and the absorbance at 450 nm is shown. Calu-3 cells were cultured (85% confluence) and lysed with or without the addition of phosphatase inhibitor to the lysis buffer (phospho or nonphospho lysate, respectively). 图2:磷酸化或非磷酸化的裂解中蛋白含量和450nm吸光值之间的关系。Calu-3细胞在85%满时收样,并用加入或未加磷酸酶抑制剂的裂解液进行处理(磷酸化或非磷酸化)。

Figure 1: Constitutive phosphorylation of DDR1 in Calu-3 cells lysed in the presence of phosphatase inhibitors* (phospho lysate) is detected by PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit #7863 (upper, right). In contrast, a low level of phospho-DDR1 protein is detected in Calu-3 cells lysed in the absence of phosphatase inhibitors* (nonphospho lysate). Similar levels of total DDR1 protein from both nonphospho and phospho lysates are detected by PathScan® Total DDR1 Sandwich ELISA Kit #7845 (upper, left). Absorbance at 450 nm is shown in the top figure while corresponding western blots using a Phospho-DDR1 (Tyr792/796/797) rabbit antibody (right) or a total DDR1 rabbit antibody (left) are shown in the bottom figure. *Phosphatase inhibitors include sodium pyrophosphate, β-glycerophosphate and Na3VO4. 图1:在磷酸酶抑制剂*存在时(磷酸化的裂解液),用PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit #7863能够检测到Calu-3细胞中磷酸化DDR1的组成型表达(上,右)。相比之下,没有磷酸酶抑制剂*存在时(非磷酸化的裂解液),仅能检测到低水平的磷酸化DDR1的表达。用PathScan® Total DDR1 Sandwich ELISA Kit #7845则能在磷酸化和非磷酸化的裂解液中检测到近似水平的总DDR1的表达(上,左)。上图显示450nm处的吸光值,下图显示用抗磷酸化DDR1 (Tyr792/796/797)的兔源单克隆抗体(右)或抗总DDR1的兔源单克隆抗体(左)进行免疫印迹检测的结果。*磷酸酶抑制剂中包含sodium pyrophosphate、β-glycerophosphate和 Na3VO4。

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