首页 PathScan Phospho-DDR1 (panTyr) Sandwich ELISA Kit
1/2
产品介绍
产品信息
简单描述
The PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated DDR1 protein. A DDR1 rabbit antibody has been coated on the microwells. After incubation with cell lysates, DDR1 protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a phospho-tyrosine mouse mAb is added to detect captured tyrosine-phosphorylated DDR1 protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of DDR1 protein phosphorylated on tyrosine residues.
*Antibodies in this kit are custom formulations specific to kit.
应用
目标/特异性
Specificity/Sensitivity
PathScan Phospho-DDR1 (panTyr) Sandwich ELISA Kit detects endogenous levels of tyrosine-phosphorylated DDR1 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human
背景
背景
The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1).
1.Vogel, W. (1999) FASEB J 13 Suppl, S77-82.
2.Ferri, N. et al. (2004) Am J Pathol 164, 1575-85.
1 Kit
5 Kit
10 Kit
20 Kit
50 Kit
当前规格1件起购
预计5-6个工作日送达，快递: 免运费，若需干冰额外收费
全部商品分类
用小程序，查商品更便捷
