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PCNA (D3H8P) XP ® Rabbit mAb

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PCNA (D3H8P) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

PCNA

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCNA protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:4000 - 1:16000
Immunofluorescence (Frozen)	1:400 - 1:1600
Immunofluorescence (Immunocytochemistry)	1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400

同种型

Rabbit IgG

分子量

研究领域

癌症,细胞生物学,

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

PCNA (D3H8P) XP Rabbit mAb recognizes endogenous levels of total PCNA protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation. 1.Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res 23, 3613-20. 2.Krishna, T.S. et al. (1994) Cell 79, 1233-43. 3.Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60.

研究领域

癌症,细胞生物学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #71395.

数据库链接

Entrez-Gene ID

5111

UniProt ID

P12004

参考图片

Flow cytometric analysis of Jurkat cells using PCNA (D3H8P) XP® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from various cell lines using PCNA (D3H8P) XP® Rabbit mAb.

Immunoprecipitation of PCNA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PCNA (D3H8P) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using PCNA (D3H8P) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PCNA (D3H8P) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PCNA (D3H8P) XP® Rabbit mAb.

Confocal immunofluorescent analysis of rat small intestine using PCNA (D3H8P) XP® Rabbit mAb (green) showing staining of proliferative cells in intestinal crypts and β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of HeLa cells using PCNA (D3H8P) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).

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货号：

13110S

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