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品牌： BD Pharmingen
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对比
咨询反应种属：
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
来源宿主：
Mouse IgG1
产品介绍
产品介绍
产品信息
抗原名称
PCNA
宿主
Mouse IgG1
免疫原
Human PCNA aa. 68-230
简单描述
Progression of the mammalian cell cycle is regulated in two different ways: 1) phosphorylation and dephosphorylation of key proteins; 2) synthesis and degradation of regulatory factors. The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. Human PCNA is 262 amino acids with an apparent molecular weight of 36 kDa. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.
商品描述
24/PCNA
Progression of the mammalian cell cycle is regulated in two different ways: 1) phosphorylation and dephosphorylation of key proteins; 2) synthesis and degradation of regulatory factors. The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. Human PCNA is 262 amino acids with an apparent molecular weight of 36 kDa. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.
同种型
Mouse IgG1
克隆号
克隆 24/PCNA (RUO)
浓度
250 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
反应种属
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
目标/特异性
PCNA
背景
别名
Proliferating Cell Nuclear Antigen
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Li Y, Dowbenko D, Lasky LA. AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival. J Biol Chem. 2002; 277(13):11352-11361. (Biology: Western blot).
2. Moore JK, Scheinman RI, Bellgrau D. The identification of a novel T cell activation state controlled by a diabetogenic gene. J Immunol. 2001; 166(1):241-248. (Biology: Western blot).
3. Reilly PT, Wysocka J, Herr W. Inactivation of the retinoblastoma protein family can bypass the HCF-1 defect in tsBN67 cell proliferation and cytokinesis. Mol Cell Biol. 2002; 22(19):6767-6778. (Biology: Western blot).
4. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology: Immunofluorescence).
5. Travali S, Ku DH, Rizzo MG, Ottavio L, Baserga R, Calabretta B. Structure of the human gene for the proliferating cell nuclear antigen. J Biol Chem. 1989; 264(13):7466-7472. (Biology).
参考图片
Immunofluorescence staining of AN3 CA cells (Human endometrial adenocarcinoma; ATCC HTB-111).
Western blot analysis of PCNA on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-PCNA antibody.
Immunofluorescence staining of AN3 CA cells (Human endometrial adenocarcinoma; ATCC HTB-111).
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