BD OptiBuild™ BV786 Rat Anti-Mouse CD140a

CD140a (PDGFR a)

Rat WF, also known as Wistar Furth IgG2a, κ

Mouse PDGF Receptor α chain

The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR-α). CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult. CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells. CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain.  Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization.  The APA5 antibody has been demonstrated to block binding of  PDGF-AA to PDGFR-a-expressing cells in vitro  and to block some PDGF-mediated developmental events in vivo. The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm.  BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

APA5 The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR-α). CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult. CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells. CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain.  Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization.  The APA5 antibody has been demonstrated to block binding of  PDGF-AA to PDGFR-a-expressing cells in vitro  and to block some PDGF-mediated developmental events in vivo. The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm.  BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

Rat WF, also known as Wistar Furth IgG2a, κ

克隆 APA5 (RUO)

0.2 mg/ml

Flow cytometry (Qualified)

Mouse (Tested in Development)

CD140a (PDGFRα)

Pdgfra; Pdgfr2; PDGF-R alpha; Platelet derived growth factor receptor alpha

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(3) 1. Fruttiger M, Calver AR, Krüger WH. PDGF mediates a neuron-astrocyte interaction in the developing retina. Neuron. 1996; 17(6):1117-1131. (Clone-specific: Blocking). 2. Heldin CH. Structural and functional studies on platelet-derived growth factor. EMBO J. 1992; 11(12):4251-4259. (Biology). 3. Takakura N, Yoshida H, Kunisada T, Nishikawa S, Nishikawa SI. Involvement of platelet-derived growth factor receptor-alpha in hair canal formation. J Invest Dermatol. 1996; 107(5):770-777. (Immunogen: Blocking, ELISA, Immunohistochemistry, Inhibition, Western blot).

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