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CD31 (PECAM-1) (89C2) Mouse mAb

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CD31 (PECAM-1) (89C2) Mouse mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

CD31

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein.

宿主

Mouse

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:800 - 1:3200
Immunofluorescence (Immunocytochemistry)	1:800 - 1:3200

同种型

Mouse IgG1

分子量

130

研究领域

癌症,纤维化,免疫学和肿瘤学,神经科学,

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

CD31 (PECAM-1) (89C2) Mouse mAb detects endogenous levels of total CD31 protein. It does not cross-react with other related proteins.

Species Reactivity:

Human

敏感性

Endogenous

背景

CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). 1.Newman, P.J. (1997) J. Clin. Invest. 99, 3-8. 2.Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-72. 3.Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-24.

研究领域

癌症,纤维化,免疫学和肿瘤学,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #85873.

数据库链接

Entrez-Gene ID

5175

UniProt ID

P16284

参考图片

Confocal immunofluorescent analysis of HUVEC (left) and HeLa cells (right) using CD31 (PECAM-1) (89C2) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Western blot analysis of extracts from HUVEC cells using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunoprecipitation of CD31 from HUVEC cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is CD31 (PECAM-1) (89C2) Mouse mAb. Western blot was performed using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human smooth muscle using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded HUVEC pellets using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using CD31 (PECAM-1) (89C2) Mouse mAb.

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货号：

3528S

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