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Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Specificity/Sensitivity
Species Reactivity:
Human
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). 1.Newman, P.J. (1997) J. Clin. Invest. 99, 3-8. 2.Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-72. 3.Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-24.
参考图片
Western blot analysis of extracts from HUVEC cells using CD31 (PECAM-1) (89C2) Mouse mAb.Western免疫印迹。用CD31 (PECAM-1) (89C2) Mouse mAb研究HUVEC提取液。
Immunohistochemical analysis of paraffin-embedded HUVEC pellets using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋HUVEC沉淀。所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb。
Flow cytometric analysis of HeLa cells (red) and HUVEC (blue), using CD31 (PECAM-1) (89C2) Mouse mAb.流式细胞仪分析HeLa 细胞(红色) 和HUVEC (绿色), 所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb。
Immunohistochemical analysis of paraffin-embedded human smooth muscle using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋人平滑肌组织。所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb。
Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋人毛细血管瘤组织。所用抗体为 CD31 (PECAM-1) (89C2) Mouse mAb。
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb.免疫组织化学染色分析石蜡包埋人透明细胞癌组织。所用抗体为 CD31 (PECAM-1) (89C2) Mouse mAb。
Confocal immunofluorescent analysis of HUVEC (left) and HeLa cells (right) using CD31 (PECAM-1) (89C2) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).共聚焦免疫荧光分析HUVEC (左图) 和 HeLa 细胞(右图) 所用抗体为CD31 (PECAM-1) (89C2) Mouse mAb (绿色）。肌动蛋白微丝用DY-554 phalloidin (红色)标记。Blue pseudocolor = DRAQ5™ (DNA 荧光染料)。