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Perforin (E9F7N) Rabbit mAb

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Perforin (E9F7N) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse perforin protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Frozen)	1:50 - 1:100
Immunofluorescence (Immunocytochemistry)	1:50 - 1:100
Flow Cytometry (Fixed/Permeabilized)	1:50 - 1:200

同种型

Rabbit IgG

分子量

70-75

应用

目标/特异性

Specificity/Sensitivity

Perforin (E9F7N) Rabbit mAb recognizes endogenous levels of total mouse perforin protein.

Species Reactivity:

Mouse

敏感性

Endogenous

背景

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells. 1.Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014. 2.Lord, S.J. et al. (2003) Immunol. Rev. 193, 31-8. 3.Trapani, J.A. and Sutton, V.R. (2003) Curr. Opin. Immunol. 15, 533-43. 4.Catalfamo, M. and Henkart, P.A. (2003) Curr Opin Immunol 15, 522-7.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

18646

UniProt ID

P10820

参考图片

Flow cytometric analysis of A20 cells (blue, negative) and CTLL-2 cells (green, positive) using Perforin (E9F7N) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from C2C12 cells, CTLL-2 cells, and mouse activated CD8+ T cells using Perforin (E9F7N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Confocal immunofluorescent analysis of mouse spleen using Perforin (E9F7N) Rabbit mAb (yellow), CD4 (RPA-T4) Mouse mAb (FITC Conjugate) #48705 (green), and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Confocal immunofluorescent analysis of CTLL-2 cells (left, positive) or BA/F3 cells (right, negative) using Perforin (E9F7N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

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货号：

44865T

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询价

20μl

100ul

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