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Pfu DNA Polymerase Ⅱ
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咨询90 kDa (Reducing)
90 kDa (Reducing)
Storage Solution : 5 U/ul Pfu DNA Polymerase Ⅱ, 20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C
10* Reaction Buffer: 200 mM Tris-HCl (pH 8.8) , 20 mM MgSO4, 100 mM KCl, 100 mM (NH4) 2SO4, 1% Triton X-100, 1 mg/ml nuclease-free BSA
2μg (R: reducing condition, N: non-reducing condition).
Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.
Store at -25 ~ -15℃ for 2 years
[1] Lin, T. C. , et al. "Cloning and expression of T4 DNA polymerase." Proceedings of the National Academy of Sciences 84.20(1987):7000-7004.
[2] Yan, Wang , et al. "A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro." Nucleic Acids Research 3(2004):1197.