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phi29 DNA Polymerase
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咨询68kDa (Reducing)
68kDa (Reducing)
10U/μl phi29 DNA Polymerase、100 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.5% Tween® 20、50% Glycerol、0.5% NP40、pH 7.4 @ 25°C
Reaction buffer: 50 mM Tris-HCl、10 mM MgCl2、10 mM (NH4)2SO4、4 mM DTT、(pH 7.5 @ 25°C)
2μg (R: reducing condition, N: non-reducing condition).
phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29 (Φ29) (1). On the one hand, the enzyme has excellent strand replacement and sustained synthesis capabilities, enabling the unstranding and replication of complex DNA structures and isothermal DNA polymerization reactions in vitro that do not depend on thermal cycling. On the other hand, the enzyme possesses 3´→5´ nucleic acid exonuclease proofreading activity.
Store at -25 ~ -15℃ for 2 years
1. Tadas P, Gediminas A , Rasa S ,et al. In vitro evolution of phi29 DNA polymerase using isothermal compartmentalized self-replication technique[J].Protein Engineering Design & Selection, 2016(12):617-628.
2. Margarita S, Isabel H, Redrejo-Rodríguez Modesto,et al. DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication[J].Frontiers in Molecular Bioences, 2016, 3:37.