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简单描述
This Phosphatase Inhibitor Cocktail is used to prevent dephosphorylation of phosphorylated proteins from active serine/threonine and tyrosine phosphatases present in whole cell extract. The 100X cocktail is a colorless to light yellow liquid.
商品描述
Product Usage Information
Briefly vortex the Phosphatase Inhibitor Cocktail (100X) before use. Then just prior to use:For Western Blot or Immunoprecipitation Cell Lysis: Dilute the cocktail 1:100 in desired lysis buffer to obtain a 1X working concentration.For PTMScan(R) Cell Lysis: Dilute the cocktail 1:50 in urea lysis buffer to obtain a 2X working concentration. This higher concentration is required due to the concentrated nature of the lysate.
应用
实验应用
W/IP
背景
背景
Dynamic protein phosphorylation is a key cellular signaling mechanism by which a broad spectrum of cellular processes is regulated. In order to study the phosphorylation status of specific target proteins the phosphorylated residue of interest must remain intact. When cells are lysed to make whole cell extracts, a loss of normal cellular signaling regulation occurs, and phosphatases within the cell extract are free to dephosphorylate proteins in an uncontrolled manner. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption.
翻译后修饰
unmodified
制备和贮存
保存方式
Store the undiluted 100X cocktail at 4ºC. Do not freeze.
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