Phospho-4E-BP1 (Ser65) Matched Antibody Pair

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

简单描述

The Phospho-4E-BP1 (Ser65) Matched Antibody Pair is ideal for use with immunoassay technologies and high-throughput ELISA platforms requiring antibody pairs with specialized or custom antibody labeling. Labels include fluorophores, lanthanides, biotin, and beads. Platforms requiring conjugated Matched Antibody Pairs include MSD, Quanterix Simoa, Alpha Technology (AlphaScreen, AlphaLISA, LANCE, HTRF), and Luminex.

Learn how Matched Antibody Pairs move your projects forward, faster at cst-science.com/matched-antibody-pairs.

商品描述

Product Usage Information

Matched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions/concentrations should be determined by the end user.

背景

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). 1.Pause, A. et al. (1994) Nature 371, 762-7. 2.Brunn, G.J. et al. (1997) Science 277, 99-101. 3.Gingras, A.C. et al. (1998) Genes Dev 12, 502-13. 4.Fadden, P. et al. (1997) J Biol Chem 272, 10240-7. 5.Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

翻译后修饰

phosphate

制备和贮存

保存方式

Store at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

数据库链接

Entrez-Gene ID

1978

UniProt ID

Q13541

参考图片

Data using Phospho-4E-BP1 (Ser65) Matched Antibody Pair #99459 are shown. The two antibodies function as a capture-target-detection sandwich (#42235 and #27899, respectively) to detect endogenous protein expression in cells treated with amino acid (AA) replenishment and untreated cells.

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货号：

99459S

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