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Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.
免疫组织化学分析石蜡包埋的NCI-H2228细胞，未经处理（左）或苯乙双胍处理（右），使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗。
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.
免疫组织化学分析石蜡包埋的人肺癌细胞，未经处理（左）或λ磷酸酶处理（右），使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗。
Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.
Western blot方法检测SH-SY5Y细胞提取物：未处理或使用Oligomycin #9996 处理(0.5 μM, 30 min)，使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗 （上图）或 Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676（下图）。使用λ 磷酸酶验证抗体的磷酸化特异性。
Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
共聚焦免疫荧光分析293细胞（所有饥饿处理为Krebs-Ringer碳酸氢盐缓冲液处理4hr），仅饥饿（左上），血清处理（10%, 30min；右上），过氧化氢处理（10 mM, 10min；左下），或λ磷酸酶处理（2hr，右下），用Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗（绿色）（Ser79）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。
