老带新
Phospho-Akt (Ser473) Antibody
1/7

Phospho-Akt (Ser473) Antibody

分享
品牌: CST
pdf 下载产品说明书
pdf 下载COA
pdf 下载SDS
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
分子量:
60
反应种属:
Human,Mouse,Rat,Hamster,Monkey,D. melanogaster,Bovine,Dog,
展开
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Akt
来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of mouse Akt. Antibodies are purified by protein A and peptide affinity chromatography.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunoprecipitation1:100
Immunofluorescence (Immunocytochemistry)1:50 - 1:200
Flow Cytometry (Fixed/Permeabilized)1:100 - 1:400
分子量
60
内毒素水平
猴, 鸡 , 非洲爪蟾蜍, 马
研究领域
癌症,细胞生物学,纤维化,代谢,神经科学
应用
反应种属
Human,Mouse,Rat,Hamster,Monkey,D. melanogaster,Bovine,Dog,
预测反应种属
Monkey,Chicken,Xenopus,Horse,
目标/特异性

Specificity/Sensitivity

Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. It does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or p70 S6 kinase.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Bovine, Dog

敏感性
Endogenous
背景
背景
Akt, also referred to as PKB or Rac, plays a critical role in controlling cell survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3K/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin-dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). 1.Franke, T.F. et al. (1997) Cell 88, 435-7. 2.Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602. 3.Franke, T.F. et al. (1995) Cell 81, 727-36. 4.Alessi, D.R. et al. (1996) EMBO J 15, 6541-51. 5.Sarbassov, D.D. et al. (2005) Science 307, 1098-101. 6.Jacinto, E. et al. (2006) Cell 127, 125-37. 7.Cardone, M.H. et al. (1998) Science 282, 1318-21. 8.Brunet, A. et al. (1999) Cell 96, 857-68. 9.Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4. 10.Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 11.Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8. 12.Hajduch, E. et al. (2001) FEBS Lett 492, 199-203. 13.Cross, D.A. et al. (1995) Nature 378, 785-9. 14.Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. 15.Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30. 16.Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52. 17.Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. 18.Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. 19.Manning, B.D. et al. (2002) Mol Cell 10, 151-62. 20.Devi, L. and Ohno, M. (2015) Transl Psychiatry 5, e562.
研究领域
癌症,细胞生物学,纤维化,代谢,神经科学
翻译后修饰
phosphate
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

数据库链接
Entrez-Gene ID
208,10000,207
UniProt ID
P31751,Q9Y243,P31749

参考图片

Flow cytometric analysis of LNCaP cells, untreated (green) or LY294002-treated (blue), using Phospho-Akt (Ser473) Antibody compared to a nonspecific negative control antibody (red).

Western blot analysis of extracts from SK-MEL-28, NIH/3T3, and H-4-II-E cells, untreated (-) or treated (+) as indicated with: human Insulin (hInsulin; 100 nM, 20 min), mouse PDGF-BB (mPDGF-BB; 100 ng/mL, 20 min), or human IGF-1 (hIGF-1; 100 ng/mL, 5 min) using Phospho-Akt (Ser473) Antibody (upper) or Akt (pan) (C67E7) Rabbit mAb  #4691 (lower).

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with PDGF, wortmannin, LY294002, rapamycin or PD98059, using Phospho-Akt (Ser473) Antibody.

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with PDGF for the indicated times, using Phospho-Akt (Ser473) Antibody (upper) or Akt Antibody #9272 (lower).

Western blot analysis of immunoprecipitated Akt from 293 cells transiently transfected with HA-tagged Akt (WT), HA-tagged K179A mutant Akt and HA-tagged K179A/S473A mutant Akt, using Phospho-Akt (Ser473) Antibody (upper), Akt antibody (middle) or HA antibody (lower). Phospho-Akt (Ser473) Antibody does not recognize Akt with an alanine substituion at Ser473. (Polakiewicz, R.D. et al. [1998] J. Biol. Chem. 273, 23534-23541.)

Immunoprecipitation of phospho-Akt (Ser473) from C2C12 cell extracts. Cells were treated with Insulin (100 ng/mL, 10 min.). Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is Phospho-Akt (Ser473) Antibody. Western blot analysis was performed using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

Confocal immunofluorescent analysis of C2C12 cells, treated with LY294002 #9901 (50 uM, 2 hrs; left) or insulin-treated (100 ng/mL, 30 min; right), using Phospho-Akt (Ser473) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
货号:
9271S
一键复制
询价
优宁维现货
20ul
100ul
300ul
选择数量
当前规格1件起购 
配送至
预计3-4个工作日送达,快递: 免运费,若需干冰额外收费