







参考图片
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with PDGF, wortmannin, LY294002, rapamycin or PD98059, using Phospho-Akt (Ser473) Antibody.
Western blot 分析NIH/3T3 细胞提取物, 非处理组和PDGF, wortmannin, LY294002, rapamycin 或PD98059处理组,所用抗体为 Phospho-Akt (Ser473) Antibody。
Western blot analysis of immunoprecipitated Akt from 293 cells transiently transfected with HA-tagged Akt (WT), HA-tagged K179A mutant Akt and HA-tagged K179A/S473A mutant Akt, using Phospho-Akt (Ser473) Antibody (upper), Akt antibody (middle) or HA antibody (lower). Phospho-Akt (Ser473) Antibody does not recognize Akt with an alanine substituion at Ser473. (Polakiewicz, R.D. et al. [1998] J. Biol. Chem. 273, 23534-23541.)
Western blot 分析瞬时转染HA-tagged Akt (WT), HA-tagged K179A突变Akt 和 HA-tagged K179A/S473A 突变 Akt的293 细胞免疫沉淀的Akt,所用抗体为Phospho-Akt (Ser473) Antibody (上), Akt antibody (中) or HA antibody (下)。 Phospho-Akt (Ser473) Antibody 不能识别丙氨酸代换的Akt。(Polakiewicz, R.D. et al. [1998] J. Biol. Chem. 273, 23534-23541.)
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with PDGF for the indicated times, using Phospho-Akt (Ser473) Antibody (upper) or Akt Antibody #9272 (lower).
Western blot分析NIH/3T3细胞的提取物,分为未处理组和PDGF处理组,如图中的时间所示,使用的抗体是Phospho-Akt (Ser473) Antibody (上) 或者 Akt Antibody #9272 (下)。
Flow cytometric analysis of LNCaP cells, untreated (green) or LY294002-treated (blue), using Phospho-Akt (Ser473) Antibody compared to a nonspecific negative control antibody (red).
流式细胞术分析未处理(绿色)或者LY294002处理(蓝色)的LNCaP细胞,使用的抗体是Phospho-Akt (Ser473) Antibody,并和非特异性阴性对照抗体比较(红色)。
Confocal immunofluorescent images of C2C12 cells serum starved and treated with or without insulin as indicated and labeled with Phospho-Akt (Ser473) Antibody (red). Actin filaments have been labeled with fluorescein phalloidin.
免疫荧光共聚焦分析C2C12细胞,如图分别为血清饥饿,胰岛素未处理或者处理组,并应用Phospho-Akt (Ser473) 抗体标记(红色)。肌动蛋白纤维由荧光素-鬼笔环肽(fluorescein phalloidin)所标记。