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Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb
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Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb

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反应种属:
All
来源宿主:
Rabbit
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Akt Substrate
来源纯化

Monoclonal antibody is produced by immunizing animals with an Akt substrate peptide library.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Peptide ELISA (DELFIA)1:1000
同种型
Rabbit
应用
反应种属
All
目标/特异性

Specificity/Sensitivity

Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb recognizes endogenous proteins containing phospho-Ser/Thr preceded by Arg at positions -5 and -3 in a manner largely independent of the surrounding amino acid sequence. Minor cross-reactivity is observed for proteins that contain phospho-Ser/Thr preceded by Arg at position -3 only. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs.

Species Reactivity:

All Species Expected

敏感性
Endogenous
背景
背景
An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. 1.Montminy, M. (1997) Annu Rev Biochem 66, 807-22. 2.Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3.Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8. 4.Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53. 5.Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4. 6.Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

参考图片

Western blot analysis of extracts from A-431 cells, untreated or treated with Human Epidermal Growth Factor (hEGF) #8916 (10 ng/ml, 20 min), using Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb (upper), or Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (lower). Western blot images were obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology).

Western blot analysis of extracts from MKN-45 cells, untreated or treated with c-Met kinase inhibitor SU11274 (1 µM, 2 hr), using Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb. Western blot image was obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology).

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货号:
10001S
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20μl
100ul
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