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Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb

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Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1289 of human HER3/ErbB3.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:50 - 1:250
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:1000

同种型

Rabbit

分子量

185

应用

目标/特异性

Specificity/Sensitivity

Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb detects endogenous HER3/ErbB3 proteins only when phosphorylated at tyrosine 1289. This antibody cross-reacts with overexpressed EGFR. This antibody may cross-react with overexpressed receptor tyrosine kinases in IHC.

Species Reactivity:

Human, Mouse

敏感性

Endogenous

背景

HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but it lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins, and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3K (4).Investigators have found that ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). Research studies have demonstrated that in tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g., ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, investigators view inhibiting interaction between ErbB3 and ErbB tyrosine kinases as a novel strategy for anti-tumor therapy. 1.Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37. 2.Guy, P.M. et al. (1994) Proc Natl Acad Sci U S A 91, 8132-6. 3.Songyang, Z. et al. (1993) Cell 72, 767-78. 4.Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55. 5.Sithanandam, G. et al. (2003) Carcinogenesis 24, 1581-92. 6.Kobayashi, M. et al. (2003) Oncogene 22, 1294-301. 7.Holbro, T. et al. (2003) Proc Natl Acad Sci U S A 100, 8933-8.

研究领域

癌症,细胞生物学,

翻译后修饰

phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #73367.

数据库链接

Entrez-Gene ID

2065

UniProt ID

P21860

参考图片

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, untreated (left) or Calf Intestinal Phosphatase (CIP)-treated (right), using Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb.

Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb specifically binds to phosphorylated HER3/ErbB3 but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).

Western blot analysis of extracts from mIMCD-3 cells, untreated or treated with neuregulin-1 (100 ng/ml, 5 min) using Phospho-HER3/ErbB3 (Tyr1289) (21D3) RmAb.

Simple Western™ analysis of lysates (0.1 mg/mL) MCF-7 +hNRG1 (100ng/mL, 30 mins) cells using Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb #4791. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded MDA-MB-453 cells, untreated (left) or neuregulin-treated (right), using Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb.

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货号：

4791T

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