首页 抗体 一抗 一抗 Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit mAb (Alexa Fluor ® 647 Conjugate)
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产品介绍
产品信息
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human Stat6 protein.
简单描述
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit mAb #56554.
商品描述
Product Usage Information
| Application | Dilution |
|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
应用
目标/特异性
Specificity/Sensitivity
Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit mAb (Alexa Fluor 647 Conjugate) recognizes endogenous levels of Stat6 protein only when phosphorylated at Tyr641. Weak cross-reactivity with other tyrosine-phosphorylated proteins has been observed in some cell lines.
Species Reactivity:
Human, Mouse
背景
背景
Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
1.Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2.Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3.Hou, J. et al. (1994) Science 265, 1701-1706.
4.Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5.Takeda, K. et al. (1996) Nature 380, 627-630.
6.Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7.Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.
制备和贮存
保存方式
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
参考图片
Flow cytometric analysis of ACHN cells, untreated (blue) or treated with hIL-4 (10 ng/mL, 10 min; green), using Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit mAb (Alexa Fluor® 647 Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (dashed line).
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