PI3 Kinase Antibody Sampler Kit

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PI3 Kinase Antibody Sampler Kit

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

PI3 Kinase

简单描述

The PI3 Kinase Sampler Kit provides an economical means of studying PI3 kinase subunits in cells. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

背景

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). 1.Cantley, L.C. (2002) Science 296, 1655-7. 2.Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. 3.Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. 4.Stoyanov, B. et al. (1995) Science 269, 690-3.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

phosphate

数据库链接

Entrez-Gene ID

5289,5290,5295,8503,5291,5294,5296

UniProt ID

Q8NEB9,P42336,P27986,Q92569,P42338,P48736,O00459

参考图片

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from MCF-7 and K-562 cells using PI3 Kinase p110β (C33D4) Rabbit mAb.

Simple WesternTM analysis of lysates (1 mg/ml) from K-562 cells lysates using PI3 Kinase p110β (C33D4) Rabbit mAb #3011. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230kDa.

Western blot analysis of extracts from 293 and RN33B cells using PI3 Kinase Class III (D4E2) Rabbit mAb.

Simple Western™ analysis of lysates (1 mg/mL) from 293 cells using PI3 Kinase Class III (D4E2) Rabbit mAb #3358. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Western blot analysis of extracts from NIH/3T3-Src cells, untreated or treated with lambda phosphatase and from C2C12 cells, untreated or treated with H2O2, using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody.

Immunoprecipitation of NIH/3T3-Src cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody. Western blot was performed using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody.

Simple Western™ analysis of lysates (1 mg/mL) from 3T3 cells using PI3 Kinase p110α (C73F8) Rabbit mAb #4249 . The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Western blot analysis of extracts from various cell lines using PI3 Kinase p110α (C73F8) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from Jurkat and NIH/3T3 cells, using PI3 Kinase p85 (19H8) Rabbit mAb.

Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb.

Immunoprecipitation of PI3 Kinase p110α protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is PI3 Kinase p110α (C73F8) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Western blot analysis was performed using PI3 Kinase p110α (C73F8) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

Simple Western™ analysis of lysates (1 mg/mL) from 3T3 cells using PI3 Kinase p85 (19H9) Rabbit mAb #4257. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Western blot analysis of extracts from NIH/3T3, Jurkat and K562 cells using PI3 Kinase p110γ (D55D5) Rabbit mAb.

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货号：

9655T

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