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PI3 Kinase p110alpha (C73F8) Rabbit mAb

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PI3 Kinase p110alpha (C73F8) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

PI3 Kinase p110alpha

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Asp520 of the sequence of human PI3K p110α.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50

同种型

Rabbit IgG

分子量

110

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Bovine,

目标/特异性

Specificity/Sensitivity

PI3 Kinase p110α (C73F8) Rabbit mAb detects endogenous levels of total PI3K p110α protein.

Species Reactivity:

Human, Mouse, Rat, Bovine

敏感性

Endogenous

背景

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). 1.Cantley, L.C. (2002) Science 296, 1655-7. 2.Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. 3.Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. 4.Stoyanov, B. et al. (1995) Science 269, 690-3.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

5290

UniProt ID

P42336

参考图片

Immunoprecipitation of PI3 Kinase p110α protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is PI3 Kinase p110α (C73F8) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Western blot analysis was performed using PI3 Kinase p110α (C73F8) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

Western blot analysis of extracts from various cell lines using PI3 Kinase p110α (C73F8) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb.

Simple Western™ analysis of lysates (1 mg/mL) from 3T3 cells using PI3 Kinase p110α (C73F8) Rabbit mAb #4249 . The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

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货号：

4249S

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