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Human PIK3R4 MAb (Clone 576514) (25 ug)

品牌： R&D Systems

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产品介绍

产品信息

荧光素标记

Unconjugated

纯化方式

Protein A or G purified from hybridoma culture supernatant

抗原名称

EC 2.7.11.1， MGC102700， p150， phosphatidylinositol 3-kinase-associated p150， Phosphoinositide 3-kina

宿主

Mouse IgG2a

免疫原

E. coli-derived recombinant human PIK3R4
Gln58-Arg230
Accession # Q99570

克隆号

576514

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PIK3R4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PIK3R4 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 576514

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PIK3R4
Gln58-Arg230
Accession # Q99570

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PIK3R4 antibody by Western Blot.

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Detection of Human PIK3R4 by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human PIK3R4 Monoclonal Antibody (Catalog # MAB6104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PIK3R4 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIK3R4

PIK3R4 (phosphoinositide 3-kinase regulatory subunit 4) is a 150 kDa, 1358 amino acid (aa) adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway. It is a regulatory subunit of the PI3K complex and is ubiquitously expressed. Within aa 58‑230, human PIK3R4 shares 99% aa identity with mouse and rat PIK3R4.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 4

Entrez Gene IDs

30849 (Human); 75669 (Mouse); 363131 (Rat)

Alternate Names

EC 2.7.11.1; MGC102700; p150; phosphatidylinositol 3-kinase-associated p150; Phosphoinositide 3-kinase adaptor protein; phosphoinositide 3-kinase regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4, p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; PIK3R4; VPS 15; VPS15

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Apoptosis Intracellular Kinases
Diabetic Peripheral Neuropathy
Epithelial Cell Polarity
Intracellular Kinases
Intracellular Kinases in the Akt Pathway
Search Kinases and Phosphatases

应用

实验应用

Western Blot(1 µg/mL)

背景

别名

EC 2.7.11.1,MGC102700,p150,phosphatidylinositol 3-kinase-associated p150,Phosphoinositide 3-kinase adaptor protein,phosphoinositide 3-kinase regulatory subunit 4,phosphoinositide-3-kinase, regulatory subunit 4,phosphoinositide-3-kinase, regulatory subunit 4, p150,PI3-kinase p150 subunit,PI3-kinase regulatory subunit 4,PIK3R4,VPS 15,VPS15,Phosphoinositide-3-kinase, Regulatory Subunit 4

背景

Background: PIK3R4

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 4

Entrez Gene IDs

30849 (Human); 75669 (Mouse); 363131 (Rat)

Alternate Names

研究领域

Related Research Areas

Apoptosis Intracellular Kinases
Diabetic Peripheral Neuropathy
Epithelial Cell Polarity
Intracellular Kinases
Intracellular Kinases in the Akt Pathway
Search Kinases and Phosphatases

制备和贮存

溶解方法

Reconstitute at 0.5 mg/mL in sterile PBS.

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

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货号：

MAB6104-SP

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