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产品介绍
产品介绍
产品信息
抗原来源
Human
宿主
Mouse
免疫原
Purified recombinant protein fragment corresponding to aa39-181 from human PLCG1, expressed in E. coli.
产品用途
Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.
简单描述
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi where it activates Ras. This protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
同种型
IgG2a
克隆号
2F3D11
克隆类别
Monoclonal
纯度
Purified
形式
Supplied as a liquid in PBS, 0.05% sodium azide.
应用
实验应用
E FC WB
推荐用量
ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
反应种属
Mouse
交叉反应性
Hu Rt
目标/特异性
Recognizes human PLCG1. Species Crossreactivity: rat
稀释度
ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
背景
别名
PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
制备和贮存
保存方式
Storage_Temp:-20°C
Storage_and_Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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